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Open Access Research

Lentiviral-Mediated Transgene Expression Can Potentiate Intestinal Mesenchymal-Epithelial Signaling

Adria D Dismuke1, Aimee D Kohn2, Randall T Moon3 and Melissa H Wong4*

Author Affiliations

1 Department of Molecular and Medical Genetics, Oregon Health & Science University, Portland, OR, USA

2 Cascade Cancer Center, Kirkland, WA, USA

3 Howard Hughes Medical Institute, Division of Hematology, Department of Pharmacology, and the Center for Developmental Biology, Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA, USA

4 Departments of Dermatology; Cell and Developmental Biology; Knight Cancer Institute, Oregon Health & Science University, Portland, OR, USA

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Biological Procedures Online 2009, 11:130-144  doi:10.1007/s12575-009-9014-z

Published: 14 July 2009

Abstract

Mesenchymal-epithelial signaling is essential for the development of many organs and is often disrupted in disease. In this study, we demonstrate the use of lentiviral-mediated transgene delivery as an effective approach for ectopic transgene expression and an alternative to generation of transgenic animals. One benefit to this approach is that it can be used independently or in conjunction with established transgenic or knockout animals for studying modulation of mesenchymal-epithelial interactions. To display the power of this approach, we explored ectopic expression of a Wnt ligand in the mouse intestinal mesenchyme and demonstrate its functional influence on the adjacent epithelium. Our findings highlight the efficient use of lentiviral-mediated transgene expression for modulating mesenchymal-epithelial interactions in vivo.

Keywords:
lentivirus; mesenchymal-epithelial signaling; intestine; transgene expression; Wnt signaling; mouse