Table 2 |
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|
LDH absorbance changes at 340 nm as a function of time in the presence or absence of Rottlerin |
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|
DMSO |
20 μM Rottlerin |
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|
|
|
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|
Time |
OD |
ΔA |
OD |
ΔA |
|
|
||||
|
30 s |
1.248 |
1.365 |
||
|
1.5 min |
1.211 |
0.037 |
1.320 |
0.045 |
|
2.5 min |
1.180 |
0.031 |
1.296 |
0.024 |
|
3.5 min |
1.147 |
0.033 |
1.262 |
0.034 |
|
4.5 min |
1.122 |
0.025 |
1.222 |
0.040 |
|
Mean ΔA/min |
0.031 |
0.035 |
||
|
LDH (U/l) |
503 |
568 |
||
|
|
||||
|
The values reported in the table are representative of two LDH assays in the same sample and conditions |
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|
Maioli et al. Biological Procedures Online 2009 11:227 doi:10.1007/s12575-009-9020-1 |
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