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Media composition influences yeast one- and two-hybrid results

Ying Liu1, Zabeena Merchant1, Hao-Ching Hsiao2, Kim L Gonzalez2, Kathleen S Matthews1 and Sarah E Bondos12*

Author Affiliations

1 Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005 USA

2 Department of Molecular and Cellular Medicine, Texas A&M University, College Station, TX, 77843 USA

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Biological Procedures Online 2011, 13:6  doi:10.1186/1480-9222-13-6

Published: 15 August 2011


Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may optimize signal differences for individual experiments, media preparation must be reported in detail to replicate studies and accurately compare results from different experiments.

Yeast one-hybrid; yeast two-hybrid; protein-protein interaction; accuracy; false positive; false negative