A method for non-invasive genotyping of APCmin/+ mice using fecal samples
1 Nutrigenomics and Nutrigenetics, CSIRO- Food and Nutritional Sciences, Adelaide, South Australia
2 Nerve-Gut Research Laboratory, Royal Adelaide Hospital, Level 1 IMVS Building, Frome Road, Adelaide, South Australia, 5000
Biological Procedures Online 2012, 14:1 doi:10.1186/1480-9222-14-1Published: 30 January 2012
The APCmin/+ mouse is commonly used in cancer research and is just one of many genetically altered models that is currently being developed. With high numbers of breeding programs, it is important to have a simple method that can be used to genotype the mice non-invasively. Here we report a reproducible method for genotyping mice with DNA extracted from fecal samples. Comparison of fecal results with those obtained from intestinal tissue DNA and clinical outcome (presence/absence of tumors) showed this technique to have 100% accuracy. This non-invasive method of genotyping may be applied to other transgenic mouse models.