ATP concentration dependence of 45Ca2+ uptake in ER microsomes isolated from male rat brain. Net 45Ca2+ accumulation was measured by liquid scintillation counting after the uptake reaction was allowed to proceed for 60 minutes at 37°C in buffer containing 300 nM free Ca2+ and 3 mM Mg2+ (see materials and methods). Data are means ± SEM, n = 3.
McMullen et al. Biological Procedures Online 2012 14:4 doi:10.1186/1480-9222-14-4