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Open Access Research

Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

Michael Flanagan1, Jeffrey M Gimble2, Gang Yu2, Xueqing Xia3, Bruce A Bunnell4 and Shulin Li35*

Author Affiliations

1 Department of Comparative Biomedical Sciences, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, USA

2 Pennington Biomedical Research Center, 6400 Perkins Rd., Baton Rouge, LA 70808, USA

3 Department of Pediatrics Research, The University Texas MD Anderson Cancer Center, Graduate School of Biomedical Sciences, 1515 Holcombe, Houston, TX, USA

4 Director, Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, USA

5 Department of Pediatrics Research, The University of Texas MD Anderson Cancer Center, The University of Texas Graduate School of Biomedical Sciences at Houston, Houston, TX 77030, USA

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Biological Procedures Online 2012, 14:7  doi:10.1186/1480-9222-14-7

Published: 18 April 2012

Abstract

Background

Adipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use.

Results

We developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells.

Conclusions

Our findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation.

Keywords:
Electroporation; Formulation; Stem cells; Transfection; Cell therapy