First two steps in the selection of electroporation formulation for human adipose-derived stem cells (ASCs). Error bars expressed as mean × SEM (n = 3). *, indicating a significant difference was detected at p < 0.05. (a) Step 1 of buffer optimization. 1 × 10E6 cells were used for each cell transfection in 100 μL indicated buffers and different programs with use of Amaxa nucleofector. (b) Step 2 of buffer optimization. The key for polymer abbreviations is in the Methods section. Differentiation of each polymer was tested. None, optimum buffer without addition of any polymer.
Flanagan et al. Biological Procedures Online 2012 14:7 doi:10.1186/1480-9222-14-7