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Methodology   Open Access

An evaluation of the reliability of muscle fiber cross-sectional area and fiber number measurements in rat skeletal muscle

Lisa Ceglia, Sathit Niramitmahapanya, Lori L Price, Susan S Harris, Roger A Fielding, Bess Dawson-Hughes Biological Procedures Online 2013, 15:6 (8 March 2013)

Abstract | Full text | PDF | PubMed

Research   Open Access

Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods

Tatiana Sedlackova, Gabriela Repiska, Peter Celec, Tomas Szemes, Gabriel Minarik Biological Procedures Online 2013, 15:5 (13 February 2013)

Abstract | Full text | PDF | PubMed

Review   Open Access

Next generation sequencing in cancer research and clinical application

Derek Shyr, Qi Liu Biological Procedures Online 2013, 15:4 (13 February 2013)

Abstract | Full text | PDF | PubMed

Methodology   Open Access Highly Accessed

A do-it-yourself protocol for simple transcription activator-like effector assembly

Claudia Uhde-Stone, Nilang Gor, Tiffany Chin, Joseph Huang, Biao Lu Biological Procedures Online 2013, 15:3 (14 January 2013)

Abstract | Full text | PDF | PubMed

Research   Open Access

Determine the quality of human embryonic stem colonies with laser light scattering patterns

Chi-Shuo Chen, Matthew Biasca, Catherine Le, Eric Y-T Chen, E Daniel Hirleman, Wei-Chun Chin Biological Procedures Online 2013, 15:2 (14 January 2013)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A new method of kidney biopsy using low dose CT-guidance with coaxial trocar and bard biopsy gun

Xiao-ling Pi, Zhen Tang, Li-qian Fu, Mei-hua Guo, Mei-hua Shi, Lan Chen, Zheng-ying Wan Biological Procedures Online 2013, 15:1 (7 January 2013)

Abstract | Full text | PDF | PubMed

Short report   Open Access

Spontaneous arthritis and ankylosis in male DBA/1 mice: further evidence for a role of behavioral factors in “stress-induced arthritis”

Kirsten Braem, Shea Carter, Rik J Lories Biological Procedures Online 2012, 14:10 (19 December 2012)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

Modified protocol for in vivo imaging of wild-type mouse retina with customized miniature spectral domain optical coherence tomography (SD-OCT) device

Lee R Ferguson, Sankarathi Balaiya, Sandeep Grover, Kakarla V Chalam Biological Procedures Online 2012, 14:9 (11 October 2012)

Abstract | Full text | PDF | PubMed

Short report   Open Access Highly Accessed

A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors

Claudia Uhde-Stone, Joseph Huang, Biao Lu Biological Procedures Online 2012, 14:8 (8 August 2012)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Research   Open Access

Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells

Michael Flanagan, Jeffrey M Gimble, Gang Yu, Xueqing Xia, Bruce A Bunnell, Shulin Li Biological Procedures Online 2012, 14:7 (18 April 2012)

Abstract | Full text | PDF | PubMed

Research   Open Access

Novel mouse mammary cell lines for in vivo bioluminescence imaging (BLI) of bone metastasis

Celeste Bolin, Caleb Sutherland, Ken Tawara, Jim Moselhy, Cheryl L Jorcyk Biological Procedures Online 2012, 14:6 (17 April 2012)

Abstract | Full text | PDF | PubMed

Research   Open Access

A reinvestigation of somatic hypermethylation at the PTEN CpG island in cancer cell lines

Luke B Hesson, Deborah Packham, Emily Pontzer, Pauline Funchain, Charis Eng, Robyn L Ward Biological Procedures Online 2012, 14:5 (10 April 2012)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

David C McMullen, William S Kean, Ajay Verma, Jeffrey T Cole, William D Watson Biological Procedures Online 2012, 14:4 (2 April 2012)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

An improved competitive inhibition enzymatic immunoassay method for tetrodotoxin quantification

Amber N Stokes, Becky L Williams, Susannah S French Biological Procedures Online 2012, 14:3 (12 March 2012)

Abstract | Full text | PDF | PubMed

Research   Open Access

Enhancement of anti-murine colon cancer immunity by fusion of a SARS fragment to a low-immunogenic carcinoembryonic antigen

Chen-Si Lin, Shih-Han Kao, Yu-Cheng Chen, Chi-Han Li, Yuan-Ting Hsieh, Shang-Chih Yang, Chang-Jer Wu, Ru-Ping Lee, Kuang-Wen Liao Biological Procedures Online 2012, 14:2 (3 February 2012)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A method for non-invasive genotyping of APCmin/+ mice using fecal samples

Erin L Symonds, Michael Fenech Biological Procedures Online 2012, 14:1 (30 January 2012)

Abstract | Full text | PDF | PubMed

Short report   Open Access

Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes

Cecilia Mancini, Erika Messana, Emilia Turco, Alessandro Brussino, Alfredo Brusco Biological Procedures Online 2011, 13:10 (28 October 2011)

Abstract | Full text | PDF | PubMed

Methodology   Open Access Highly Accessed

An automated cell-counting algorithm for fluorescently-stained cells in migration assays

Baraa K Al-Khazraji, Philip J Medeiros, Nicole M Novielli, Dwayne N Jackson Biological Procedures Online 2011, 13:9 (19 October 2011)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

Rapid generation of long tandem DNA repeat arrays by homologous recombination in yeast to study their function in mammalian genomes

Vladimir N Noskov, Nicholas CO Lee, Vladimir Larionov, Natalay Kouprina Biological Procedures Online 2011, 13:8 (7 October 2011)

Abstract | Full text | PDF | PubMed

Research   Open Access Highly Accessed

Frequency shifting approach towards textual transcription of heartbeat sounds

Farshad Arvin, Shyamala Doraisamy, Ehsan Safar Khorasani Biological Procedures Online 2011, 13:7 (4 October 2011)

Abstract | Full text | PDF | PubMed |  Editor’s summary

A novel frequency-shifting method for recording heartbeat sounds in text format increases the accuracy of transcription, providing useful information for monitoring the behaviour of patients with cardiovascular problems.

Short report   Open Access Highly Accessed

Media composition influences yeast one- and two-hybrid results

Ying Liu, Zabeena Merchant, Hao-Ching Hsiao, Kim L Gonzalez, Kathleen S Matthews, Sarah E Bondos Biological Procedures Online 2011, 13:6 (15 August 2011)

Abstract | Full text | PDF | PubMed

Research   Open Access Highly Accessed

A rapid and easy method for the DNA extraction from Cryptococcus neoformans

Fatma Mseddi, Mohammed Jarboui, Amira Sellami, Hayet Sellami, Ali Ayadi Biological Procedures Online 2011, 13:5 (21 July 2011)

Abstract | Full text | PDF | PubMed

Research   Open Access Highly Accessed

Quantitative Assessment of Mammary Gland Density in Rodents Using Digital Image Analysis

John N McGinley, Henry J Thompson Biological Procedures Online 2011, 13:4 (10 June 2011)

Abstract | Full text | PDF | PubMed

Methodology   Open Access Highly Accessed

A quantitative PCR method for measuring absolute telomere length

Nathan J O'Callaghan, Michael Fenech Biological Procedures Online 2011, 13:3 (31 January 2011)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Editorial   Open Access

Biological Procedures Online now publishing with BioMed Central

Shulin Li Biological Procedures Online 2011, 13:2 (31 January 2011)

Abstract | Full text | PDF | PubMed

Research   Open Access Highly Accessed

Influence of RT-qPCR primer position on EGFR interference efficacy in lung cancer cells

Gang Chen, Peter Kronenberger, Erik Teugels, Jacques De Grève Biological Procedures Online 2011, 13:1 (11 November 2010)

Abstract | Full text | PDF

Methodology   Open Access

Utilization of IκB–EGFP Chimeric Gene as an Indicator to Identify Microbial Metabolites with NF-κB Inhibitor Activity

Yu-Ling Lin, Yen-Shun Chen, Jui-Hung Hsieh, Ching-Min Lin, Hsin-Yi Wu, Chen-Si Lin, Rea-Min Chu, Kuang-Wen Liao, Yuan-Hsun Hsu Biological Procedures Online 2010, 12:131-138 (2 October 2010)

Abstract | Full text | PDF

Methodology   Open Access

A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis

John N McGinley, Matthew D Thompson, Henry J Thompson Biological Procedures Online 2010, 12:118-130 (18 May 2010)

Abstract | Full text | PDF

Methodology   Open Access

A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip

Holger Weishaupt, Joanne L Attema Biological Procedures Online 2010, 12:1-17 (15 May 2010)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A New Microsphere-Based Immunoassay for Measuring the Activity of Transcription Factors

Yu-Ling Lin, Yun-Ju Lai, Nu-Man Tsai, Tai-Chu Peng, Yen-Ku Liu, Ru-Ping Lee, Chueh-Jen Tsai, Kuang-Wen Liao Biological Procedures Online 2010, 12:18-26 (14 April 2010)

Abstract | Full text | PDF

Methodology   Open Access

Quick and Simple Detection Technique to Assess the Binding of Antimicrotubule Agents to the Colchicine-Binding Site

Sébastien Fortin, Jacques Lacroix, Marie-France Côté, Emmanuel Moreau, Éric Petitclerc, René C-Gaudreault Biological Procedures Online 2010, 12:113-117 (8 April 2010)

Abstract | Full text | PDF | PubMed

Research   Open Access

Highly Efficient Stable Expression of Indoleamine 2,3 Dioxygenase Gene in Primary Fibroblasts

Alireza Rezakhanlou, Darya Habibi, Amy Lai, Reza B Jalili, Christopher J Ong, Aziz Ghahary Biological Procedures Online 2010, 12:107-112 (27 March 2010)

Abstract | Full text | PDF

Methodology   Open Access

Optimized RNA Extraction and Northern Hybridization in Streptomycetes

Marcello Tagliavia, Anna Taravella, Sandra Marineo, Anna Puglia, Mario La Farina Biological Procedures Online 2010, 12:27-30 (17 March 2010)

Abstract | Full text | PDF

Research   Open Access

Collection of Epithelial Cells from Rodent Mammary Gland Via Laser Capture Microdissection Yielding High-Quality RNA Suitable for Microarray Analysis

John N McGinley, Zongjian Zhu, Weiqin Jiang, Henry J Thompson Biological Procedures Online 2010, 12:31-43 (3 March 2010)

Abstract | Full text | PDF

Research   Open Access

Comparison between Culture Conditions Improving Growth and Differentiation of Blood and Bone Marrow Cells Committed to the Endothelial Cell Lineage

Claudio Muscari, Chiara Gamberini, Ilaria Basile, Francesca Bonafé, Simond Valgimigli, Ombretta Capitani, Carlo Guarnieri, Claudio Caldarera Biological Procedures Online 2010, 12:89-106 (6 February 2010)

Abstract | Full text | PDF | PubMed

Review   Open Access

Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Nakul Mandal, Steffen Heegaard, Jan Prause, Bent Honoré, Henrik Vorum Biological Procedures Online 2010, 12:56-88 (24 December 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf

Ning Wu, Kanyand Matand, Sonya Williams Biological Procedures Online 2010, 12:44-55 (24 December 2009)

Abstract | Full text | PDF | PubMed

Research   Open Access

Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs

Anita Gothelf, Pernille Hojman, Julie Gehl Biological Procedures Online 2009, 11:325-330 (3 December 2009)

Abstract | Full text | PDF | PubMed

Review   Open Access Highly Accessed

A Practical Guide to Rodent Islet Isolation and Assessment

Jeffrey D Carter, Stacey B Dula, Kathryn L Corbin, Runpei Wu, Craig S Nunemaker Biological Procedures Online 2009, 11:3-31 (3 December 2009)

Abstract | Full text | PDF | PubMed

Research   Open Access

Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers

Emanuela Maioli, Claudia Torricelli, Vittoria Fortino, Filippo Carlucci, Valentina Tommassini, Adriana Pacini Biological Procedures Online 2009, 11:227-240 (3 December 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A Modified Coupled Enzyme Method for O-linked GlcNAc Transferase Activity Assay

Lianwen Zhang, Feifei Ren, Jing Li, Xiaofeng Ma, Peng Wang Biological Procedures Online 2009, 11:170-183 (3 December 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1

Andrea Staršíchová, Lukáš Kubala, Eva Lincová, Zuzana Pernicová, Alois Kozubík, Karel Souček Biological Procedures Online 2009, 11:316-324 (12 September 2009)

Abstract | Full text | PDF

Editorial   Open Access

The Possible Cellular Mechanism for Extending Lifespan of Mice with Rapamycin

Shulin Li Biological Procedures Online 2009, 11:1-2 (1 September 2009)

Full text | PDF | PubMed

Research   Open Access

MicroRNA Expression Profiling by Bead Array Technology in Human Tumor Cell Lines Treated with Interferon-Alpha-2a

Fredy Siegrist, Thomas Singer, Ulrich Certa Biological Procedures Online 2009, 11:113-129 (23 July 2009)

Abstract | Full text | PDF | PubMed | Cited on BioMed Central

Methodology   Open Access

Segmentation of Extrapulmonary Tuberculosis Infection Using Modified Automatic Seeded Region Growing

Iman Avazpour, M Iqbal Saripan, Abdul Nordin, Raja Syamsul Azmir Raja Abdullah Biological Procedures Online 2009, 11:241-252 (14 July 2009)

Abstract | Full text | PDF | PubMed

Research   Open Access

Lentiviral-Mediated Transgene Expression Can Potentiate Intestinal Mesenchymal-Epithelial Signaling

Adria D Dismuke, Aimee D Kohn, Randall T Moon, Melissa H Wong Biological Procedures Online 2009, 11:130-144 (14 July 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples

Jane J Pappas, André Toulouse, WEC Bradley Biological Procedures Online 2009, 11:99-112 (24 June 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

A Method for Structure–Activity Analysis of Quorum-Sensing Signaling Peptides from Naturally Transformable Streptococci

XiaoLin Tian, Raymond T Syvitski, TianLei Liu, Nadine Livingstone, David L Jakeman, Yung-Hua Li Biological Procedures Online 2009, 11:207-226 (11 June 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access Highly Accessed

A Framework for White Blood Cell Segmentation in Microscopic Blood Images Using Digital Image Processing

Farnoosh Sadeghian, Zainina Seman, Abdul Ramli, Badrul Abdul Kahar, M-Iqbal Saripan Biological Procedures Online 2009, 11:196-206 (11 June 2009)

Abstract | Full text | PDF | PubMed

Methodology   Open Access

SDS-PAGE-Based Quantitative Assay for Screening of Kidney Stone Disease

Lau Wai-Hoe, Leong Wing-Seng, Zhari Ismail, Gam Lay-Harn Biological Procedures Online 2009, 11:145-160 (15 May 2009)

Abstract | Full text | PDF | PubMed

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