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Open Access Methodology

Modified protocol for in vivo imaging of wild-type mouse retina with customized miniature spectral domain optical coherence tomography (SD-OCT) device

Lee R Ferguson, Sankarathi Balaiya, Sandeep Grover and Kakarla V Chalam*

Author Affiliations

Department of Ophthalmology, University of Florida College of Medicine, 580 W 8th Street, Tower 2, 3rd floor, Jacksonville, FL 32209, USA

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Biological Procedures Online 2012, 14:9  doi:10.1186/1480-9222-14-9

Published: 11 October 2012

Abstract

This protocol outlines and evaluates a modified scanning procedure for a customized spectral domain optical coherence tomography (SD-OCT) imaging apparatus within the wild-type C57Bl/6 mouse posterior segment. This modified protocol allows for the capture of a 50 degree field of view spanning 3 mm by 3 mm perimeter with the optic disc as the central point. By utilizing this scanning protocol a more reliable measurement of retinal thickness can be achieved outside the fluctuating region of the optic disc. This protocol, when applied to this high resolution device, enables non-invasive in vivo histological imaging and biometric assessment of the various layers of the rodent posterior segment within a 20 – 30 min procedural time-frame. This protocol could establish a standardized method for evaluating morphological changes, with this commercial SDOCT device, when assessing longitudinal disease pathophysiology and treatment response in mouse models for future vision science research.

Keywords:
Spectral domain optical coherence tomography; Customized; Retinal imaging; Mouse